Tomar R H, Taylor F B
Biochem J. 1971 Dec;125(3):793-802. doi: 10.1042/bj1250793.
Reactions between purified plasminogen and streptokinase, labelled with (131)I and (125)I respectively, were investigated by polyacrylamide-gel discontinuous electrophoresis. A molecular complex consisting of both (131)I-labelled plasminogen and (125)I-labelled streptokinase migrated between plasminogen and streptokinase. This complex contained bovine plasminogen activator activity. The relative quantities of (131)I-labelled plasminogen and (125)I-labelled streptokinase in this complex were markedly affected by reaction conditions. A fragment that retained 50% or more of the parent activator activity was released from the complex after exposure to mercaptoethanol. This subcomponent had an estimated molecular weight of 70000, and contained both (131)I-labelled plasminogen and (125)I-labelled streptokinase.
分别用（131）I和（125）I标记的纯化纤溶酶原与链激酶之间的反应，通过聚丙烯酰胺凝胶不连续电泳进行研究。由（131）I标记的纤溶酶原和（125）I标记的链激酶组成的分子复合物在纤溶酶原和链激酶之间迁移。该复合物具有牛纤溶酶原激活剂活性。反应条件对该复合物中（131）I标记的纤溶酶原和（125）I标记的链激酶的相对量有显著影响。暴露于巯基乙醇后，从复合物中释放出保留50%或更多母体激活剂活性的片段。该亚组分的估计分子量为70000，并且同时含有（131）I标记的纤溶酶原和（125）I标记的链激酶。
