Isenberg G, Giesbrecht P, Wecke J, Wohlfahrt-Bottermann K E
Microsc Acta. 1975 May;77(1):30-6.
Cytoplasmic actomyosin fibrils of Physarum polycephalum were demonstrated by the freeze-etching technique. Within the fibrils the electron micrographs reveal 40-80 A thick filaments presumably representing F-actin. Thicker filaments (myosin) could not be detected. To avoid freezing artefacts, a pretreatment of the living material with 50% sucrose was necessary. The influence of freeze-protecting agents upon the fine structure of cytoplasmic actomyosins and the resulting difficulties in the investigation of constituents of the groundplasm with the freeze-etching technique are discussed.
利用冷冻蚀刻技术证实了多头绒泡菌的细胞质肌动球蛋白纤维。在这些纤维内部，电子显微镜照片显示出40 - 80埃厚的细丝，推测代表F - 肌动蛋白。未检测到更粗的细丝（肌球蛋白）。为避免冷冻假象，有必要用50%的蔗糖对活体材料进行预处理。讨论了冷冻保护剂对细胞质肌动球蛋白精细结构的影响以及用冷冻蚀刻技术研究基本质成分时由此产生的困难。
