Sadkov A I, Piliashenko-Novokhatnyĭ A I, Belova V S, Dibtsov V P, Gvozdev R I
Prikl Biokhim Mikrobiol. 1979 Jul-Aug;15(4):627-32.
The reaction vessel has been designed to measure methane monooxygenase activity. An elastic membrane has been built into one of the walls of the vessel to take liquid samples, avoiding formation of the gaseous phase in the reaction volume. The methane content in the samples is measured in a gas-liquid chromatograph with a flame ionization detector in two ways: 1. by direct measurement of methane in the liquid sample, and 2. by measurement of methane in the gaseous phase after methane diffusion from the liquid sample into the gaseous space of another vessel. The method is simple, sensitive (with a lower limit of 0.1 nMole CH4), and well reproducible. This method permits measurement of the oxidation kinetics of methane and other gaseous hydrocarbons both by intact cells and cell-free preparations of methane oxidizing bacteria.
反应容器已设计用于测量甲烷单加氧酶活性。容器的其中一面壁上内置了一个弹性膜，用于采集液体样品，避免在反应体积内形成气相。样品中的甲烷含量通过带有火焰离子化检测器的气液色谱仪以两种方式进行测量：1. 通过直接测量液体样品中的甲烷；2. 通过测量甲烷从液体样品扩散到另一个容器的气相空间后其中的甲烷。该方法简单、灵敏（下限为0.1纳摩尔CH4）且重现性良好。此方法允许通过完整细胞和甲烷氧化细菌的无细胞制剂来测量甲烷及其他气态烃的氧化动力学。
