柔红霉素代谢：柔红霉素还原酶的评估
Daunorubicin metabolism: estimation of daunorubicin reductase.
作者信息
Bachur N R, Huffman D H
出版信息
Br J Pharmacol. 1971 Dec;43(4):828-33. doi: 10.1111/j.1476-5381.1971.tb07219.x.
Abstract
- Daunorubicin reductase, an enzyme ubiquitous in all mammalian tissues tested, converts daunorubicin, a cancer chemotherapeutic antibiotic, to daunorubicinol. A method for the estimation of daunorubicinol and daunorubicin reductase is described.2. Daunorubicin and daunorubicinol were quantitatively extracted from enzyme assay mixtures with 0.3 N HCl in 50% ethanol and then completely hydrolysed to their respective aglycones. The aglycones were extracted, concentrated into toluene, separated by silicic acid microcolumn chromatography, and estimated by spectrofluorimetry.3. Compared to previous methods, this method is highly reproducible and sensitive.
摘要
- 柔红霉素还原酶是一种在所测试的所有哺乳动物组织中普遍存在的酶，它可将抗癌化疗抗生素柔红霉素转化为柔红霉醇。本文描述了一种测定柔红霉醇和柔红霉素还原酶的方法。2. 用含0.3N盐酸的50%乙醇从酶分析混合物中定量提取柔红霉素和柔红霉醇，然后将它们完全水解为各自的苷元。提取苷元，浓缩至甲苯中，通过硅酸微柱色谱法分离，并用荧光分光光度法进行测定。3. 与以前的方法相比，该方法具有高度的可重复性和敏感性。
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Daunorubicin metabolism: estimation of daunorubicin reductase.柔红霉素代谢：柔红霉素还原酶的评估
Br J Pharmacol. 1971 Dec;43(4):828-33. doi: 10.1111/j.1476-5381.1971.tb07219.x.
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FATTY ACID AMIDES OF ETHANOLAMINE IN MAMMALIAN TISSUES.哺乳动物组织中的乙醇胺脂肪酸酰胺
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Daunorubicinol, a major metabolite of daunorubicin: isolation from human urine and enzymatic reactions.柔红霉素醇，柔红霉素的主要代谢产物：从人尿中分离及酶促反应
J Pharmacol Exp Ther. 1971 Jun;177(3):573-8.
3
The pharmacokinetics of daunomycin in man.柔红霉素在人体中的药代动力学。
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Tissue distribution and disposition of daunomycin (NCS-82151) in mice: fluorometric and isotopic methods.柔红霉素（NCS - 82151）在小鼠体内的组织分布与处置：荧光法和同位素法
Cancer Chemother Rep. 1970 Apr;54(2):89-94.
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Daunomycin metabolism in rat tissue slices.柔红霉素在大鼠组织切片中的代谢
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Daunorubicin metabolism by rat tissue preparations.
J Pharmacol Exp Ther. 1971 Jun;177(3):567-72.
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