Welsh I R, Spitznagel J K
Infect Immun. 1971 Aug;4(2):97-102. doi: 10.1128/iai.4.2.97-102.1971.
Separation of homogenates of human polymorphonuclear leukocytes (PMN) into different fractions by sedimentation in centrifugal fields that ranged from 126 x g to 50,000 x g resulted in a differential distribution of the lysosomal enzymes. Peroxidase, lysozyme, beta-glucuronidase, and acid phosphatase activity were separated from each other. This demonstrates that the lysosomes of human PMN comprise at least three and possibly four physically and chemically different cytoplasmic particles. Proteins which are more cationic than lysozyme and which may be analogous to cationic lysosomal protein of rabbit PMN were associated with lysozyme and beta-glucuronidase rich granules. Antibacterial activity was present in four of the five cell fractions which this work produced. These results are significant because they differ from those obtained with rabbits and because they directly influence future experimental design and interpretation, in attempts to analyze antibacterial, scavenging, and inflammatory capacities of human PMN. Since lysosomes differ physically, biochemically, and morphologically, they may well differ with respect to their function in the PMN.
通过在126×g至50,000×g的离心场中沉降，将人多形核白细胞（PMN）匀浆分离成不同组分，导致溶酶体酶的差异分布。过氧化物酶、溶菌酶、β-葡萄糖醛酸酶和酸性磷酸酶活性彼此分离。这表明人PMN的溶酶体至少包含三种，可能四种物理和化学性质不同的细胞质颗粒。比溶菌酶更具阳离子性且可能类似于兔PMN阳离子溶酶体蛋白的蛋白质与富含溶菌酶和β-葡萄糖醛酸酶的颗粒相关。这项工作产生的五个细胞组分中有四个具有抗菌活性。这些结果很重要，因为它们与用兔子获得的结果不同，并且因为它们直接影响未来的实验设计和解释，以便分析人PMN的抗菌、清除和炎症能力。由于溶酶体在物理、生化和形态上存在差异，它们在PMN中的功能很可能也不同。
