Behm H L, Wagner J G
Res Commun Chem Pathol Pharmacol. 1979 Oct;26(1):145-60.
In equilibrium dialysis protein binding experiments, the fraction of free or bound drug determined at equilibrium by dialysis of a patient plasma sample does not correspond to the drug concentration in the initial plasma sample, but to the drug concentration inside the plasma compartment of the dialysis apparatus at equilibrium. That fraction of free or bound drug corresponds to a lower total drug concentration than that in the initial plasma sample because of loss of drug due to passage of free drug into the buffer compartment in the equilibrium dialysis experiment. The magnitude of the difference between the initial drug concentration and the equilibrium drug concentration depends on the extent of drug binding and experimental conditions. If the initial total drug concentration in the plasma or serum sample as well as the fraction of drug bound at equilibrium as determined by radiotracer methods are known, then, using methods given in the text, the equilibrium drug concentration, the binding parameters corresponding to equilibrium conditions, and the free drug concentration in the initial plasma sample may be estimated.
在平衡透析蛋白结合实验中，通过对患者血浆样本进行透析在平衡时测定的游离或结合药物分数，并非对应于初始血浆样本中的药物浓度，而是对应于平衡时透析装置血浆隔室内的药物浓度。由于在平衡透析实验中游离药物进入缓冲隔室导致药物损失，该游离或结合药物分数对应的总药物浓度低于初始血浆样本中的总药物浓度。初始药物浓度与平衡药物浓度之间差异的大小取决于药物结合程度和实验条件。如果已知血浆或血清样本中的初始总药物浓度以及通过放射性示踪剂方法测定的平衡时结合药物分数，那么使用文中给出的方法，可以估算平衡药物浓度、对应平衡条件的结合参数以及初始血浆样本中的游离药物浓度。
