Khrapunov S N, Berdyshev G D
Ukr Biokhim Zh (1978). 1979 Sep-Oct;51(5):467-73.
The efficiency of intramolecular and intermolecular interactions of histons H1, H2b and H4 was studied as affected by the glycine concentration in the composition of the glycine-HCl buffer (pH 3.0). It is shown that the concentration of glycine less than 0.01m should be used in experiments with the glycine buffer. Glycine in a higher concentration evokes a considerable aggregation of the histon molecules. Sizes and shape of the aggregates differ in solutions with the lower and high ionic strength. In the presence of glycine the sizes of histone aggregates decrease in the series H1 greater than H2b greater than H4. Changes in the quantum yield of histon fluorescence reflect changes in the tertiary structure of their molecule but not in aggregation.
研究了甘氨酸 - HCl缓冲液（pH 3.0）组成中甘氨酸浓度对组蛋白H1、H2b和H4分子内和分子间相互作用效率的影响。结果表明，在使用甘氨酸缓冲液的实验中，应使用浓度低于0.01m的甘氨酸。较高浓度的甘氨酸会引起组蛋白分子的大量聚集。在低离子强度和高离子强度的溶液中，聚集体的大小和形状有所不同。在甘氨酸存在的情况下，组蛋白聚集体的大小按H1大于H2b大于H4的顺序减小。组蛋白荧光量子产率的变化反映了其分子三级结构的变化，而不是聚集情况的变化。
