Shevriakov N V, Babiĭchuk A M, Iavonenko A F
Ukr Biokhim Zh (1978). 1979 Sep-Oct;51(5):497-502.
In the cattle rumen mucosa the citrate synthesis from alpha-ketoglutarate and CO2 is activated by ATP and biotin; in the muscular tissue ATP or biotin separately does not affect this process. Mn2+, Zn2+, Mg2+, Co2+, Cd2+ and cystein favour citrate synthesis; citrate is not formed in the presence of Fe2+. For homogenates prepared on the borate buffer, the rate of an increase in the citrate content is higher than for homogenates on the phosphate or tris-HCl-buffer. In the muscular tissue of the lamb rumen this index is greater than in the sheep, and in the mucosa cattle, sheep and lamb the intensity of the reaction is the same.
在牛瘤胃黏膜中，由α-酮戊二酸和二氧化碳合成柠檬酸的过程被ATP和生物素激活；在肌肉组织中，单独的ATP或生物素不会影响这一过程。锰离子、锌离子、镁离子、钴离子、镉离子和半胱氨酸有利于柠檬酸的合成；在亚铁离子存在的情况下不会形成柠檬酸。对于在硼酸盐缓冲液中制备的匀浆，柠檬酸含量的增加速率高于在磷酸盐或三羟甲基氨基甲烷盐酸盐缓冲液中的匀浆。在羔羊瘤胃的肌肉组织中，该指标高于绵羊，而在牛、绵羊和羔羊的黏膜中，反应强度相同。
