小牛晶状体β-晶状体蛋白半胱氨酸残基周围的氨基酸序列。
Amino acid sequences around the cysteine residue of calf lens -crystallin.
作者信息
Corran P H, Waley S G
出版信息
Biochem J. 1971 Aug;124(1):61-7. doi: 10.1042/bj1240061.
Abstract
- Calf lens alpha-crystallin was carboxymethylated with radioactive sodium iodoacetate to label the thiol group. 2. The protein was then digested with trypsin or alternatively fractionated in urea to obtain the acidic (A) chains, which were then digested with trypsin. Either procedure gave two radioactive peptides containing carboxymethylcysteine. 3. These two peptides were closely related: the longer form contained 28 amino acid residues, and the shorter lacked two residues at the N-terminal end of the longer form. 4. The amino acid sequence of the peptides have been determined. 5. No evidence for the presence of more than one cysteine residue/chain was found. 6. The question of the molecular weight of the chains is discussed.
摘要
- 用放射性碘乙酸钠对小牛晶状体α-晶状体蛋白进行羧甲基化，以标记巯基。2. 然后用胰蛋白酶消化该蛋白质，或者在尿素中进行分级分离以获得酸性（A）链，随后用胰蛋白酶消化这些链。这两种方法都得到了两条含有羧甲基半胱氨酸的放射性肽段。3. 这两条肽段密切相关：较长的形式含有28个氨基酸残基，较短的形式在较长形式的N末端缺少两个残基。4. 已经确定了这些肽段的氨基酸序列。5. 未发现每条链存在一个以上半胱氨酸残基的证据。6. 讨论了这些链的分子量问题。
相似文献
1
Amino acid sequences around the cysteine residue of calf lens -crystallin.小牛晶状体β-晶状体蛋白半胱氨酸残基周围的氨基酸序列。
Biochem J. 1971 Aug;124(1):61-7. doi: 10.1042/bj1240061.
2
Structural studies on bovine -crystallin.牛β-晶状体蛋白的结构研究。
Biochem J. 1971 Feb;121(3):453-9. doi: 10.1042/bj1210453.
3
The amino acid sequence around four cysteine residues in trout actin.鳟鱼肌动蛋白中四个半胱氨酸残基周围的氨基酸序列。
Biochem J. 1971 Jul;123(4):591-600. doi: 10.1042/bj1230591.
4
Structural studies on lens proteins.晶状体蛋白的结构研究。
Biochem J. 1967 Jul;104(1):128-34. doi: 10.1042/bj1040128.
5
The amino acid sequence of -crystallin (fraction II) from calf lens.来自小牛晶状体的β-晶状体蛋白（组分II）的氨基酸序列。
Biochem J. 1972 Jul;128(4):961-70. doi: 10.1042/bj1280961.
6
Amino acid sequences containing cysteine or cystine residues in ovalbumin from eggs of the quail Coturnix coturnix japonica.来自日本鹌鹑（Coturnix coturnix japonica）蛋的卵清蛋白中含有半胱氨酸或胱氨酸残基的氨基酸序列。
Aust J Biol Sci. 1981;34(5-6):505-14. doi: 10.1071/bi9810505.
7
The structure of the acidic polypeptide chains from alpha-crystallin. Amino acid composition, peptide mapping, and N-terminus.α-晶状体蛋白酸性多肽链的结构。氨基酸组成、肽图谱及N端。
Int J Protein Res. 1971;3(1):19-24. doi: 10.1111/j.1399-3011.1971.tb01688.x.
8
Structural studies of alpha-crystallin.α-晶状体蛋白的结构研究。
Biochem J. 1965 Sep;96(3):722-8. doi: 10.1042/bj0960722.
9
-Crystallin. Fractionation of subunits and sequence studies on an isolated polypeptide.-晶状体蛋白。亚基的分级分离及对一种分离出的多肽的序列研究。
Biochem J. 1971 Sep;124(2):345-55. doi: 10.1042/bj1240345.
10
N-terminal sequences of alpha-crystallin.α-晶状体蛋白的N端序列
Biochem J. 1969 Dec;115(4):789-96. doi: 10.1042/bj1150789.
本文引用的文献
1
PREFERENTIAL RELEASE OF ASPARTIC ACID BY DILUTE ACID TREATMENT OF TRYPTIC PEPTIDES.
Biochemistry. 1965 May;4:793-801. doi: 10.1021/bi00881a001.
2
THE DEAGGREGATION OF BOVINE LENS ALPHA-CRYSTALLIN.牛晶状体α-晶体蛋白的解聚作用。
J Biol Chem. 1965 May;240:1979-85.
3
Splitting and recombination of alpha-crystallin.α-晶状体蛋白的分裂与重组
Exp Eye Res. 1962 Jun;1:300-5. doi: 10.1016/s0014-4835(62)80015-3.
4
The action of trypsin on polylysine.胰蛋白酶对聚赖氨酸的作用。
Biochem J. 1953 Sep;55(2):328-37. doi: 10.1042/bj0550328.
5
Structural studies on lens proteins.晶状体蛋白的结构研究。
Biochem J. 1967 Jul;104(1):128-34. doi: 10.1042/bj1040128.
6
Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups.肽在纸上的电泳迁移率及其在酰胺基团测定中的应用。
Nature. 1966 Aug 6;211(5049):591-3. doi: 10.1038/211591a0.
7
Further studies on the sub-units of alpha-crystallin.关于α-晶状体蛋白亚基的进一步研究。
Biochem J. 1966 Apr;99(1):179-88. doi: 10.1042/bj0990179.
8
Structural studies of alpha-crystallin.α-晶状体蛋白的结构研究。
Biochem J. 1965 Sep;96(3):722-8. doi: 10.1042/bj0960722.
9
The effect of denaturing agents on the molecular weight of bovine alpha-crystallin.变性剂对牛α-晶状体蛋白分子量的影响。
Biochim Biophys Acta. 1969 Aug 12;188(1):162-3. doi: 10.1016/0005-2795(69)90058-0.
10
Molecular weight determination from amino acid analysis data: a numerical method.
Anal Biochem. 1968 Oct 24;25(1):417-31. doi: 10.1016/0003-2697(68)90117-6.
Zotero 插件