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[新生大鼠大脑皮层外植体培养的形态计量学研究]

[Morphometric studies of neonatal rat cerebral cortex explant cultures].

作者信息

Goworek K

出版信息

Z Mikrosk Anat Forsch. 1979;93(3):577-85.

PMID:516857
Abstract

The following parameters were investigated quantitatively in cerebrocortex explants of rat brain after cultivation periods of 2, 4, 7, 10 and 15 days in vitro by light microscopical methods. 1. The relationship between the sizes of explants and growth area was found to be statistically significant by correlation and regression analysis of the area index calculated from growth area and explant area following cultivation periods of two and four days in vitro. A significant increase in the cultivation area was also states (exception: 7th--10th). 2. The presence and changing percentage in the number of different cell populations within the growth area showed good correlations to previous results obtained from cerebellum explants in vitro. Problems of adaptive changes of the astrocytes to the in-vitro-conditions are discussed.

摘要

通过光学显微镜方法,对大鼠脑皮质外植体在体外培养2、4、7、10和15天后的以下参数进行了定量研究。1. 通过对体外培养两天和四天后根据生长面积和外植体面积计算出的面积指数进行相关性和回归分析,发现外植体大小与生长面积之间的关系具有统计学意义。培养面积也有显著增加(例外情况:第7天至第10天)。2. 生长区域内不同细胞群体数量的存在情况和变化百分比与先前从小脑外植体体外培养获得的结果具有良好的相关性。讨论了星形胶质细胞对体外条件的适应性变化问题。

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