Lang H, Vengerov Y Y, Popenko V I, Zimmer C
Acta Biol Med Ger. 1979;38(1):33-40.
Structural changes of chromatin induced by interaction of netropsin (Nt) with DNA has been examined by analysis of CD and electromicroscopic measurements. The results demonstrate the existence of a transition from the condensed globular state of chromatin into nucleosomal fibres generated by extremely low Nt concentration up to 1 mole Nt per 200 nucleotides. A second transition occurs at high Nt ratio per DNA phosphate (v' = 0.3). involving disorganization of nucleosomal particles. The interference of the Nt binding with chromatin proteins maintaining the sub- and superstructure will be discussed.
通过圆二色性（CD）分析和电子显微镜测量，研究了纺锤菌素（Nt）与DNA相互作用诱导的染色质结构变化。结果表明，存在一种转变，即从染色质的凝聚球状状态转变为由极低浓度的Nt（每200个核苷酸中Nt浓度高达1摩尔）产生的核小体纤维。在高Nt与DNA磷酸的比例（v' = 0.3）时发生第二次转变，涉及核小体颗粒的解体。将讨论Nt与维持亚结构和超结构的染色质蛋白结合的干扰情况。
