Kerbiriou D, Griffin J H
Adv Exp Med Biol. 1979;120B:153-61.
Human high MW kininogen can be isolated as a single polypeptide chain of 110,000 MW. Purified human plasma kallikrein cleaves this molecule to give a disulfide-linked, two chain molecule, free of kinin, that retains full clotting activity. Following reduction and carboxymethylation of the two chain molecule, a light chain can be isolated that quantitatively retains the full coagulant activity of the native molecule. During contact activation in normal human plasma a rapid cleavage of high MW kininogen along with kinin liberation occurs in a reaction that is dependent upon the presence of prekallikrein and Factor XII (Hageman factor).
人高分子量激肽原可作为一条分子量为110,000的单多肽链分离出来。纯化的人血浆激肽释放酶切割该分子，产生一个无激肽的、通过二硫键连接的双链分子，该分子保留了全部凝血活性。在双链分子进行还原和羧甲基化后，可分离出一条轻链，该轻链定量保留了天然分子的全部凝血活性。在正常人血浆的接触激活过程中，高分子量激肽原会快速裂解并伴随激肽释放，这一反应依赖于前激肽释放酶和因子XII（哈格曼因子）的存在。
