过敏反应的嗜碱性粒细胞激肽释放酶（BK-A）——纯化与特性分析

Basophil kallikrein of anaphylaxis (BK-A)--purification and characterization.

作者信息

Newball H H, Berninger R W, Talamo R C, Lichtenstein L M

出版信息

Adv Exp Med Biol. 1979;120B:233-47.

Abstract

These studies describe the IgE-mediated release of a basophil kallikrein of anaphylaxis (BK-A) that has arginine esterase activity and is inhibited by plasma, DFP, and Trasylol. The interaction of BK-A active fractions from ion exchange (DEAE-Sephacel) and gel filtration (Sepharose 6B) chromatography, with human plasma kininogen generates immunoreactive kinin. The BK-A and kinin-generating activities co-chromatograph on DEAE-Sephacel and Sepharose 6B columns, and the quantity of kinin generated is, in general, proportional to the BK-A activity of the column fractions, suggesting that these two activities are subserved by the same protease. These data suggest that kallikrein-like activity can be generated from human basophils as a direct result of a primary IgE-mediated immune reaction, thus providing a potential link between reactions of immediate hypersensitivity and the plasma and/or tissue kinin-generating systems.

摘要

这些研究描述了嗜碱性粒细胞激肽释放酶在过敏反应中由IgE介导的释放（BK-A），其具有精氨酸酯酶活性，并受到血浆、二异丙基氟磷酸酯（DFP）和抑肽酶的抑制。来自离子交换（DEAE-葡聚糖凝胶）和凝胶过滤（琼脂糖6B）色谱的BK-A活性组分与人血浆激肽原相互作用产生免疫反应性激肽。BK-A和激肽生成活性在DEAE-葡聚糖凝胶和琼脂糖6B柱上共同色谱分离，并且生成的激肽量通常与柱级分的BK-A活性成比例，这表明这两种活性由同一种蛋白酶承担。这些数据表明，作为主要IgE介导的免疫反应的直接结果，人嗜碱性粒细胞可产生类激肽释放酶活性，从而在速发型超敏反应与血浆和/或组织激肽生成系统之间提供了潜在联系。