Ray A C, Tamulinas S H, Reagor J C
Am J Vet Res. 1979 Apr;40(4):498-504.
Experimental animals (rabbit, rat, goat, sheep, and pony) were given cantharidin or dried preparations of blister beetles (Epicauta lemniscata) to stimulate naturally occurring toxicosis in which beetles were ingested with alfalfa hay. A sensitive high-pressure liquid chromatographic method, involving derivatization of cantharidin with p-nitrobenzyloxyamine, was developed to detect the toxin extracts of ingesta, fluids, and tissues from these severely poisoned animals. Urine and ingesta from the upper portion of the gastrointestinal tract, containing from 1 to 20 ppm of cantharidin, were the most satisfactory samples for diagnosing toxicosis. Beetle preparations also were assayed and found to contain widely varying amounts of cantharidin (0.89% to 5.40% of dry weight). Blood chemical analyses on sera and urine samples from the sheep and pony indicated renal dysfunction.
给实验动物（兔子、大鼠、山羊、绵羊和小马）服用斑蝥素或斑蝥干燥制剂（豆芫菁），以诱发自然发生的中毒，即动物在食用苜蓿干草时摄入了斑蝥。开发了一种灵敏的高压液相色谱法，该方法涉及用对硝基苄氧基胺对斑蝥素进行衍生化，用于检测这些严重中毒动物的食入物、体液和组织中的毒素提取物。胃肠道上部的尿液和食入物中斑蝥素含量为1至20 ppm，是诊断中毒最理想的样本。对斑蝥制剂也进行了分析，发现其斑蝥素含量差异很大（干重的0.89%至5.40%）。对绵羊和小马的血清及尿液样本进行的血液化学分析表明存在肾功能障碍。
