Büsen W, Hausen P
Eur J Biochem. 1975 Mar 3;52(1):179-90. doi: 10.1111/j.1432-1033.1975.tb03985.x.
Three enzymes with ribonuclease H activity are present in calf thymus. They have been separated on the basis of chromatographic behaviour and molecular weight. They are further distinguished from one another by their ionic requirements and sensitivity to the -SH reagent N-ethylmaleimide. Two of these enzymes are classified as ribonuclease H, the third is obtained in a fraction which possesses ribonuclease H activity but also degrades double-stranded RNA and poly(rA). No association between any of the enzymes and cellular DNA polymerases could be demonstrated.
小牛胸腺中存在三种具有核糖核酸酶H活性的酶。它们已根据色谱行为和分子量进行了分离。它们在离子需求和对-SH试剂N-乙基马来酰亚胺的敏感性方面也彼此有别。其中两种酶被归类为核糖核酸酶H，第三种酶存在于一个部分中，该部分不仅具有核糖核酸酶H活性，还能降解双链RNA和聚（rA）。未证明任何一种酶与细胞DNA聚合酶之间存在关联。
