从棕色固氮菌中纯化得到的细胞色素o + c4和细胞色素o的氧化还原电位。
The oxidation-reduction potentials of cytochrome o + c4 and cytochrome o purified from Azotobacter vinelandii.
作者信息
Yang T, O'Keefe D, Chance B
出版信息
Biochem J. 1979 Sep 1;181(3):763-6. doi: 10.1042/bj1810763.
Abstract
Oxidation-reduction titrations of Azotobacter vinelandii cytochrome o + c4 and cytochrome o were performed with simultaneous potential and absorbance measurements under anaerobic conditions. Cytochrome c4 has a midpoint potential (Em, 7.4) of 260mV and purified cytochrome o has an Em, 7.4 of -18mV. Little change in the midpoint potential of cytochrome o was observed when titrated in the pH range 6.2--9.8.
摘要
在厌氧条件下，对棕色固氮菌细胞色素o + c4和细胞色素o进行氧化还原滴定，并同时进行电位和吸光度测量。细胞色素c4的中点电位（Em，7.4）为260mV，纯化的细胞色素o的Em，7.4为-18mV。在pH值6.2 - 9.8范围内滴定细胞色素o时，其中点电位变化很小。
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