Yasin R, Van Beers G, Riddle P N, Brown D, Widdowson G, Thompson E J
J Cell Sci. 1979 Aug;38:201-10. doi: 10.1242/jcs.38.1.201.
The initiation of monolayer mass cell cultures from adult human biopsies has revealed a striking abnormality in the growth and development pattern of muscle cultures from Duchenne-type dystrophy. This abnormality in cell behaviour was seen as early as 4 days in culture, well before myotube formation or confluence, and consisted of areas where cells clustered together in a multilayered mass rather than showing the typical monolayer distribution normally observed. To gain some insight into the mechanism of cell cluster development, we have examined such a culture by time-lapse cinematography and also the cell behaviour of other control cultures. The results of this study show that the clusters enlarged primarily by cell division and, to a lesser extent, by the acquisition of neighbouring cells. Furthermore, none of the single cells surrounding the clusters exhibited contact inhibition of movement. This behaviour was not observed in the other cultures examined. These findings could be indicative of an abnormality in the cell surface or cell-locomotory machinery of dystrophic cells.
从成人活检组织开始进行单层大量细胞培养，已揭示出杜兴氏型肌营养不良症患者肌肉培养物在生长和发育模式上存在显著异常。这种细胞行为异常在培养4天时就已出现，远早于肌管形成或细胞汇合，表现为细胞在多层团块中聚集在一起的区域，而不是呈现通常观察到的典型单层分布。为了深入了解细胞团块发育的机制，我们通过延时摄影术研究了这样一种培养物，并研究了其他对照培养物的细胞行为。这项研究结果表明，团块主要通过细胞分裂扩大，在较小程度上通过获取邻近细胞扩大。此外，团块周围的单个细胞均未表现出运动接触抑制。在其他检测的培养物中未观察到这种行为。这些发现可能表明营养不良细胞的细胞表面或细胞运动机制存在异常。
