Sedgley M
J Cell Sci. 1979 Aug;38:49-60. doi: 10.1242/jcs.38.1.49.
Structural changes in the pollinated and unpollinated avocado (Persea americana Mill) stigma and style up to 42 h after first opening of the flower were investigated using light and electron microscopy. The pollen tubes grew in the stigma secretion and intercellular substance and initial contact occurred between the plasma membrane of the male and the cuticle and stigma secretion of the female. The pollen tube wall started to develop 15 min after pollination and increased in thickness up to 24 h after pollination. By 18 h after first opening of the flower, starch had disappeared and cell wall thickenings were present in both the pollinated and unpollinated stigma and style. The wall thickenings developed more slowly in the unpollinated than in the pollinated tissue. They contained lipid and were bounded by callose. Degeneration of the cytoplasm of some of the papilla and transmitting tissue cells occurred only following the passage of the pollen tubes and may be of importance in tube nutrition. There was no degeneration in the unpollinated stigma and style and the cytoplasm did not start to lose clarity until 42 h after first opening of the flower.
利用光学显微镜和电子显微镜，研究了鳄梨（Persea americana Mill）花朵首次开放后长达42小时内授粉和未授粉柱头及花柱的结构变化。花粉管在柱头分泌物和细胞间物质中生长，雄性质膜与雌性角质层和柱头分泌物之间开始接触。授粉后15分钟花粉管壁开始发育，授粉后24小时内厚度增加。到花朵首次开放后18小时，淀粉消失，授粉和未授粉的柱头及花柱中均出现细胞壁加厚现象。未授粉组织中细胞壁加厚的发育比授粉组织中更慢。它们含有脂质，并由胼胝质界定。一些乳头状细胞和传递组织细胞的细胞质退化仅在花粉管通过后发生，可能对花粉管营养很重要。未授粉的柱头和花柱没有退化，直到花朵首次开放后42小时细胞质才开始变得模糊不清。
