Rudd B A, Hopwood D A
J Gen Microbiol. 1979 Sep;114(1):35-43. doi: 10.1099/00221287-114-1-35.
A series of 76 mutants of Streptomyces coelicolor A3(2) specifically blocked in the synthesis of the binaphthoquinone antibiotic actinorhodin were classified into seven phenotypic classes on the basis of antibiotic activity, accumulation of pigmented precursors or shunt products of actinorhodin biosynthesis, and cosynthesis of actinorhodin in pairwise combinations of mutants. The polarity of cosynthetic reactions, and other phenotypic properties, allowed six of the mutant classes to be arranged in the most probable linear sequence of biosynthetic blocks. One member of each mutant class was mapped unambigiguously to the chromosomal linkage map in the short segment between the hisD and guaA loci, suggesting that structural genes for actinorhodin biosynthesis may form an uninterrupted cluster of chromosomal genes.
天蓝色链霉菌A3(2)的一系列76个突变体在联萘醌抗生素放线紫红素的合成中被特异性阻断，根据抗生素活性、放线紫红素生物合成的色素前体或旁路产物的积累以及突变体两两组合时放线紫红素的共合成情况，这些突变体被分为七个表型类别。共合成反应的极性以及其他表型特性，使得六个突变体类别能够按照生物合成阻断的最可能线性序列排列。每个突变体类别的一个成员被明确地定位到hisD和guaA基因座之间短片段的染色体连锁图谱上，这表明放线紫红素生物合成的结构基因可能形成一个不间断的染色体基因簇。
