Negishi K, Drujan B D
J Neurosci Res. 1979;4(5-6):335-49. doi: 10.1002/jnr.490040503.
The effect of acetylcholine (ACh) on cone- and rod-connected horizontal cells (generating photopic and scotopic L-type S-potentials, respectively) in the fish (Eugerres plumieri) retina was compared with that of dopamine (DA), and some similarities were found in the effects. About two-thirds of the L-type horizontal cells examined were sensitive to a test solution containing ACh (10 mM in concentration) and BW-anticholinesterase (BW; 1.0 mM). ACh with BW augmented a center response and attenuated a surround response as did DA (0.2 mM), although the latter effect was more pronounced and longer-lasting. ACh with BW frequently produced oscillations of the horizontal cell membrane potential, as did DA with clonidine (alpha-adrenergic stimulant). Phentolamine (alpha-adrenergic blocker) interrupted both the effects of DA and ACh on the center and surround responses, while hexamethonium (cholinergic antagonist) appeared to interfere selectively with the effect of ACh. Therefore, the ACh-induced changes observed in the horizontal cell membrane potential are assumed to be mediated by the adrenergic system in the fish retina.
将乙酰胆碱（ACh）对鱼（Eugerres plumieri）视网膜中与视锥细胞和视杆细胞相连的水平细胞（分别产生明视觉和暗视觉L型S电位）的作用与多巴胺（DA）的作用进行了比较，发现二者作用存在一些相似之处。约三分之二被检测的L型水平细胞对含有ACh（浓度为10 mM）和BW抗胆碱酯酶（BW；1.0 mM）的测试溶液敏感。ACh与BW一起增强中心反应并减弱周边反应，多巴胺（0.2 mM）也是如此，尽管后者的作用更明显且持续时间更长。ACh与BW经常使水平细胞膜电位产生振荡，多巴胺与可乐定（α-肾上腺素能兴奋剂）也是如此。酚妥拉明（α-肾上腺素能阻滞剂）可阻断多巴胺和ACh对中心和周边反应的作用，而六甲铵（胆碱能拮抗剂）似乎选择性地干扰ACh的作用。因此，在水平细胞膜电位中观察到的ACh诱导的变化被认为是由鱼视网膜中的肾上腺素能系统介导的。
