Chaĭkovskaia S M, Kushnarev V M, Tochenaia N P
Antibiotiki. 1975 Sep(9):807-10.
The paper describes the studies on definition of penicillinacylase localizations in the cells of E. coli with the help of ferritin labeled immune sera and electron microscopy. Both the intact cells and the cells treated with the substances affecting the cell wall intactness were used. The study showed relation between penicillinacylase and the surface structures of the cell, i.e. the cell wall and the periplasmic areas. It was found that penicillinacylase got into the environmental medium with the splitted cell fragments which corresponded to the general mechanism of excretion of large high molecular compounds by gramnegative organisms.
本文描述了借助铁蛋白标记免疫血清和电子显微镜对大肠杆菌细胞中青霉素酰化酶定位的研究。使用了完整细胞以及用影响细胞壁完整性的物质处理过的细胞。研究表明青霉素酰化酶与细胞的表面结构即细胞壁和周质区域之间存在关联。发现青霉素酰化酶随着对应革兰氏阴性菌排泄大分子化合物的一般机制的破碎细胞片段进入周围培养基中。
