1,25-二羟维生素D3和24,25-二羟维生素D3对实验性佝偻病的影响。

Effect of 1,25(OH)2D3 and 24,25(OH)2D3 on experimental rickets.

作者信息

Kraft D, Offermann G, Steldinger R, Gawlik D

出版信息

Res Commun Chem Pathol Pharmacol. 1979 Dec;26(3):571-80.

Abstract

The vitamin D3 metabolite 1,25(OH)2D3 alone may not be able to reverse defective bone mineralization, while a combination with another metabolite, 24,25(OH)2D3 might be necessary to display the known effect of vitamin D on bone. Growing rachitic rats were treated with 25 ng vitamin D3/d, 10 ng 1,25(OH)2D3/d, 10 ng 24,25(OH)2D3/d, 5 ng 1,25(OH)2D3/d and 5 ng 24,25(OH)2D3/d in combination and 10 ng 1,25(OH)2D3/d and 10 ng 24,25(OH)2D3/d in combination. After 10 days of treatment the epiphyseal plate width of femura and the distance of tetracycline fluorescence bands were measured microscopically on undecalcified sections of the bone. The calcium content of femur epiphysis was measured by neutron activation analysis. Neither parameter showed on greater effect of the combination of 1,25(OH)2D3 and 24,25(OH)2D3 than of 1,25(OH)2D3 alone.

摘要

仅维生素D3代谢物1,25(OH)2D3可能无法逆转有缺陷的骨矿化，而与另一种代谢物24,25(OH)2D3联合使用可能对于发挥维生素D对骨骼的已知作用是必要的。对生长中的佝偻病大鼠分别给予以下处理：每天25 ng维生素D3、每天10 ng 1,25(OH)2D3、每天10 ng 24,25(OH)2D3、每天5 ng 1,25(OH)2D3与5 ng 24,25(OH)2D3联合使用、每天10 ng 1,25(OH)2D3与10 ng 24,25(OH)2D3联合使用。治疗10天后，在未脱钙的骨切片上显微镜下测量股骨的骨骺板宽度和四环素荧光带的距离。通过中子活化分析测量股骨骨骺的钙含量。与单独使用1,25(OH)2D3相比，1,25(OH)2D3和24,25(OH)2D3联合使用在这两个参数上均未显示出更大的效果。