Kruse V
Scand J Clin Lab Invest. 1979 May;39(3):215-21. doi: 10.1080/00365517909106096.
The dissociation rate constants of a high-affinity thyroxine antiserum were estimated on the basis of curves showing the dissociation of labelled thyroxine-antibody complexes as a function of time. The dissociation curves were fitted by computer to an equation with a sum of three exponentials. The analysis of the curves gave estimates of the dissociation rate constants for fast, medium and slowly dissociating antibody populations, and also estimates of the size of the fraction of tracer bound to each of the three populations of antibodies. The coefficients of variation for the estimates were of the order of 10%, and the estimates were reproducible. The dissociation rate constants for the thyroxine antibodies were 0.55, 0.045 and 0.0058 h-1 at 23 degrees C. When labelled thyroxine and antiserum were incubated for increasing periods of time, there was an increase in the fractional binding of tracer to the slow-dissociating antibodies. The use of stripped antiserum as against unstripped antiserum also improved the fractional binding of tracer to the slow-dissociating antibodies. The stripping effect was slight for three other antisera which contained relatively fast dissociating antibodies.
基于显示标记甲状腺素 - 抗体复合物解离随时间变化的曲线，估算了一种高亲和力甲状腺素抗血清的解离速率常数。解离曲线通过计算机拟合为一个包含三个指数项之和的方程。对曲线的分析给出了快速、中等和缓慢解离抗体群体的解离速率常数估计值，以及与这三种抗体群体结合的示踪剂部分大小的估计值。估计值的变异系数约为10%，且这些估计值具有可重复性。在23摄氏度时，甲状腺素抗体的解离速率常数分别为0.55、0.045和0.0058 h⁻¹。当标记甲状腺素和抗血清孵育时间增加时，示踪剂与缓慢解离抗体的结合分数增加。使用脱蛋白抗血清相对于未脱蛋白抗血清也提高了示踪剂与缓慢解离抗体的结合分数。对于另外三种含有相对快速解离抗体的抗血清，脱蛋白效果轻微。
