Triller M, Bouratbine A, Guillaumin D, Weill R
Scan Electron Microsc. 1979(2):507-12.
Structural observations upon fluorosed enamel were made on human teeth extracted from Nabeul (Tunisia). Longitudinal sections of enamel were made and microradiographed. Topographical observatoons in the S.E.M. showed alterations of the pattern of mineralization. Fissures and clefts filled with plaque material were observed to reach to the surface of the teeth. They penetrated into enamel along the prisms. Acid etching of the surface by 30% H3PO4 revealed enlarged acid-resistant sheaths while the core of the prisms was irregularly etched. Inner portions of the enamel were observed on fractured fragments. The structure was not significantly perturbated except for areas of porosity. After demineralization of the sections by 10% EDTA at pH 7 and critical point drying, the organic matrix was observed in the S.E.M. : It appeared as a fibrous network. Compared with sound teeth the matrix was dense and irregular. Histochemical stainings showed the presence of organic material. Alcian blue and PAS positive contents were very low except near the enamel-dentine junction. Preliminary observations were made using SMI Cameca ion microanalyser and histochemical studies on undecalcified sections were made in order to compare the observations made with light microscopy with S.E.M. findings.
对从突尼斯纳布尔提取的人类牙齿的氟斑牙釉质进行了结构观察。制作了牙釉质的纵向切片并进行了显微放射照相。扫描电子显微镜（SEM）下的地形观察显示矿化模式发生了改变。观察到充满菌斑物质的裂隙和裂缝延伸至牙齿表面。它们沿着棱柱体侵入牙釉质。用30%的磷酸对表面进行酸蚀后，发现耐酸鞘增大，而棱柱体的核心被不规则蚀刻。在破碎的碎片上观察到牙釉质的内部部分。除了孔隙区域外，结构没有明显扰动。在pH值为7的条件下用10%的乙二胺四乙酸（EDTA）对切片进行脱矿并临界点干燥后，在扫描电子显微镜下观察到有机基质：它呈现为纤维状网络。与健康牙齿相比，基质致密且不规则。组织化学染色显示存在有机物质。除了在牙釉质-牙本质交界处附近，阿尔辛蓝和过碘酸希夫反应（PAS）阳性物质含量非常低。使用扫描透射离子显微镜（SMI Cameca）离子微分析仪进行了初步观察，并对未脱钙切片进行了组织化学研究，以便将光学显微镜下的观察结果与扫描电子显微镜的发现进行比较。
