Garrett J R, Parsons P A
Z Mikrosk Anat Forsch. 1979;93(4):593-608.
Normal submandibular ducts from rabbits have been examined by mucosubstance histochemistry, transmission and scanning electron microscopy. The results were compared with the appearances of ducts removed 4...6 weeks after ligation. The normal ducts were composed mainly of columnar "light" cells and basal cells but, in addition, some "dark" cells and scattered goblet containing sulphated mucins were always present. The luminal surface of the ductal cells possessed numerous microvilli protruding into the lumen, and a rim of negatively charged mucin was present on this surface of these cells. After ligation the ducts became greatly distended by their fluid contents which remained under pressure until the duct was incised. The epithelial cells were flattened and appeared to contain less cytoplasm per cell; "light" cells, basal cells and "dark" cells were still recognisable. Goblet cells were much more plentiful than in the control ducts and often protruded into the lumen despite the increased intraluminal pressure. The development of a number of ciliated cells had also occurred and they were often situated close to goblet cells. Lymphatic vessels were more prominent around the ligated ducts. Luminal microvilli were less numerous than in the control ducts but the rim of negatively charged mucin on the luminal surface of ductal cells was more conspicuous. Mixed inflammatory cells were present within the lumina of ligated ducts especially in those parts adjacent to the ductal cells. No inflammatory cell has been observed passing through the wall of a main duct and the possibility exists that these cells had entered lumina within the gland and migrated from there to the main duct. The above findings may serve to help our understanding or physiological events in the ducts.
通过黏液物质组织化学、透射电子显微镜和扫描电子显微镜对兔正常下颌下腺导管进行了检查。将结果与结扎4至6周后切除的导管外观进行了比较。正常导管主要由柱状“亮”细胞和基底细胞组成，但此外，总是存在一些“暗”细胞以及散在的含有硫酸化黏蛋白的杯状细胞。导管细胞的管腔表面有许多微绒毛伸入管腔，并且在这些细胞的该表面上存在带负电荷的黏蛋白边缘。结扎后，导管因所含液体而极度扩张，这些液体在压力下一直存在，直到切开导管。上皮细胞扁平，似乎每个细胞含有的细胞质较少；“亮”细胞、基底细胞和“暗”细胞仍可辨认。杯状细胞比对照导管中多得多，尽管管腔内压力增加，但它们常常伸入管腔。还出现了一些纤毛细胞，它们常常位于杯状细胞附近。结扎导管周围的淋巴管更明显。管腔微绒毛比对照导管中少，但导管细胞管腔表面带负电荷的黏蛋白边缘更明显。结扎导管的管腔内存在混合性炎性细胞，尤其是在与导管细胞相邻的那些部位。未观察到炎性细胞穿过主导管壁，有可能这些细胞进入腺体内的管腔并从那里迁移到主导管。上述发现可能有助于我们了解导管中的生理事件。
