Spelsberg T C, Tankersley S, Hnilica L S
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1218-25. doi: 10.1073/pnas.62.4.1218.
Arginine-rich histones (F3) interact with both the bacterial and mammalian RNA polymerase and inhibit the in vitro RNA synthesis to a much greater extent than when associated with the DNA template. The lysine-rich histones (F1) inhibit the RNA synthesis mainly through template inhibition. Neither histone fraction displayed such an interaction with DNA polymerase. The RNA polymerase-F3 histone interaction takes place at ionic strength equal to or greater than those occurring in living cells, suggesting a possible role of arginine-rich histones in the regulation of RNA synthesis.
富含精氨酸的组蛋白(F3)与细菌和哺乳动物的RNA聚合酶都相互作用,并且在体外比与DNA模板结合时更能强烈抑制RNA合成。富含赖氨酸的组蛋白(F1)主要通过抑制模板来抑制RNA合成。这两种组蛋白组分均未显示出与DNA聚合酶有此类相互作用。RNA聚合酶与F3组蛋白的相互作用发生在等于或高于活细胞内离子强度的条件下,这表明富含精氨酸的组蛋白在RNA合成调控中可能发挥作用。