利用外源DNA和RNA聚合酶选择性提取组蛋白对染色质模板活性的影响。
Effects of selective extraction of histones on template activities of chromatin by use of exogenous DNA and RNA polymerases.
作者信息
Mirsky A E, Silverman B
出版信息
Proc Natl Acad Sci U S A. 1973 Jul;70(7):1973-5. doi: 10.1073/pnas.70.7.1973.
Abstract
Exogenous DNA and RNA polymerases were used to measure the template activity of DNA in chromatin in isolated thymus nuclei from which lysinerich or arginine-rich histones were selectively extracted. Measurements were on nuclei containing 20-1200 mug of DNA per ml, the distinctions becoming clear at the higher concentrations. Experiments with RNA polymerase showed only moderate increases in template activity upon extraction of histone, although the removal of lysinerich histone had a greater effect than that of arginine-rich histone. DNA polymerase action on nuclei minus lysinerich histone achieved high results, exceeding even those on DNA itself.
摘要
使用外源性DNA和RNA聚合酶来测量从分离的胸腺细胞核中选择性提取富含赖氨酸或富含精氨酸的组蛋白后染色质中DNA的模板活性。测量对象是每毫升含有20 - 1200微克DNA的细胞核，在较高浓度时差异变得明显。用RNA聚合酶进行的实验表明，提取组蛋白后模板活性仅适度增加，尽管去除富含赖氨酸的组蛋白比去除富含精氨酸的组蛋白影响更大。DNA聚合酶对去除富含赖氨酸组蛋白的细胞核作用效果显著，甚至超过了对DNA本身的作用效果。
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