胰岛素与细胞膜的相互作用：胰岛素的主要作用。
Interaction of insulin with the cell membrane: the primary action of insulin.
作者信息
Cuatrecasas P
出版信息
Proc Natl Acad Sci U S A. 1969 Jun;63(2):450-7. doi: 10.1073/pnas.63.2.450.
Abstract
Insulin can be covalently attached to a large polymers of Sepharose through the alpha-amino group of the N-terminal residue of the B chain, or through the epsilon-amino group of its lysyl residue. Such derivatives effectively increase the utilization of glucose, and suppress the hormone-stimulated lipolysis, of isolated fat cells. The effects occur with concentrations of insulin-Sepharose that are nearly as low as those of native insulin, and the maximal responses are the same. The results indicate that interaction of insulin with superficial membrane structures alone may suffice to initiate transport as well as other metabolic alterations.
摘要
胰岛素可以通过B链N端残基的α-氨基或其赖氨酸残基的ε-氨基与琼脂糖大聚合物共价连接。这种衍生物能有效提高分离脂肪细胞对葡萄糖的利用，并抑制激素刺激的脂肪分解。在胰岛素-琼脂糖浓度几乎与天然胰岛素浓度一样低时就会出现这些效应，且最大反应相同。结果表明，胰岛素仅与表面膜结构相互作用可能就足以启动转运以及其他代谢改变。
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