压力对蛋白质表观比容的影响。

Effect of pressure on the apparent specific volume of proteins.

作者信息

Fahey P F, Kupke D W, Beams J W

出版信息

Proc Natl Acad Sci U S A. 1969 Jun;63(2):548-55. doi: 10.1073/pnas.63.2.548.

DOI:10.1073/pnas.63.2.548

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC223598/

Abstract

The magnetic densimeter has been employed to measure the densities and apparent specific volumes of certain proteins in aqueous solutions as a function of pressure. The method gave values in satisfactory agreement with those found in the literature for aqueous electrolyte solutions. A change in apparent specific volume of the monomeric proteins, ribonuclease and turnip yellow mosaic virus and its capsid protein, at pressures up to 400 atmospheres at 20 degrees C was not observed within the precision of the measurements. Also, no change in the apparent specific volume of tobacco mosaic virus protein was observed as a function of these pressures whether the protein was predominantly in the polymerized or unpolymerized state. The magnetic densimeter was found to be a convenient instrument for measuring compressibilities of very small samples of solutions.

摘要

磁密度计已被用于测量某些蛋白质水溶液的密度和表观比容随压力的变化。该方法得到的值与文献中关于水电解质溶液的值吻合良好。在20℃下高达400个大气压的压力范围内，未观察到单体蛋白核糖核酸酶、芜菁黄花叶病毒及其衣壳蛋白的表观比容有变化，测量精度范围内未发现变化。而且，无论烟草花叶病毒蛋白主要处于聚合态还是非聚合态，均未观察到其表观比容随这些压力的变化。磁密度计被发现是一种测量非常小的溶液样品压缩性的便捷仪器。

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引用本文的文献

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Simultaneous determination of viscosity and density of protein solutions by magnetic suspension.通过磁悬浮同时测定蛋白质溶液的粘度和密度

Proc Natl Acad Sci U S A. 1972 Aug;69(8):2258-62. doi: 10.1073/pnas.69.8.2258.

本文引用的文献

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Proc Natl Acad Sci U S A. 1967 Sep;58(3):888-94. doi: 10.1073/pnas.58.3.888.

2

THE NATURE OF THE COMPLEXITIES IN THE RIBONUCLEASE CONFORMATIONAL TRANSITION AND THE IMPLICATIONS REGARDING CLATHRATING.

J Am Chem Soc. 1965 Jun 20;87:2759-60. doi: 10.1021/ja01090a043.

3

POLYMERIZATION-DEPOLYMERIZATION OF TOBACCO MOSAIC VIRUS PROTEIN. IV. THE ROLE OF WATER.烟草花叶病毒蛋白的聚合-解聚。IV. 水的作用。

Biochemistry. 1965 Jan;4:31-7. doi: 10.1021/bi00877a007.

4

POLYMERIZATION-DEPOLYMERIZATION OF TOBACCO MOSAIC VIRUS PROTEIN. 3. CHANGES IN IONIZATION AND IN ELECTROPHORETIC MOBILITY.烟草花叶病毒蛋白质的聚合-解聚。3. 电离及电泳迁移率的变化

Biochemistry. 1964 Oct;3:1512-8. doi: 10.1021/bi00898a021.

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AN IMPROVED MAGNETIC DENSITOMETER: THE PARTIAL SPECIFIC VOLUME OF RIBONUCLEASE.一种改进的磁密度计：核糖核酸酶的比容

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