Jacob M, Christ B, Jacob H J
Anat Embryol (Berl). 1979;157(3):291-309. doi: 10.1007/BF00304995.
The migration of myogenic stem cells into the leg anlagen of chick embryos between stages 16--20 of Hamburger and Hamilton was examined. SEM and TEM studies reveal that cell migration starts at stage 16 from the just-formed somites 26-28. The migrating myogenic cells are elongated and oriented in a medio-lateral direction. The leading ends branch into filopodia which contact a fibrillar network. At first, single cells migrate; later on the cells leaving the ventro-lateral edge of the dermatome migrate in strands and have specialized contacts between them. After reaction with ruthenium red and concanavalin A the migrating cells show a thick surface coat to which ruthenium red-positive particles are attached. The surface coat may be important in the interactions among the migrating cells as well as between the cells and the substrate. The migration of myogenic stem cells was found to take place in a matrix of collagenous fibrils and ruthenium red-positive particles, probably containing glycosaminoglycans. At the onset of migration the fibrillar network exhibits a preferred medio-lateral orientation. Therefore, it may be concluded that this alignment of the fibrils influences the direction of cell migration.
对汉堡和汉密尔顿第16 - 20阶段鸡胚腿部原基中肌源性干细胞的迁移进行了研究。扫描电子显微镜（SEM）和透射电子显微镜（TEM）研究表明，细胞迁移从第16阶段刚形成的第26 - 28体节开始。迁移的肌源性细胞呈细长形，沿中外侧方向排列。前端分支形成丝状伪足，与纤维状网络接触。起初，单个细胞迁移；后来，从生皮节腹外侧边缘离开的细胞成束迁移，它们之间有特殊的接触。用钌红和伴刀豆球蛋白A处理后，迁移的细胞显示出一层厚厚的表面被膜，上面附着有钌红阳性颗粒。表面被膜可能在迁移细胞之间以及细胞与基质之间的相互作用中起重要作用。发现肌源性干细胞的迁移发生在胶原纤维和钌红阳性颗粒的基质中，钌红阳性颗粒可能含有糖胺聚糖。在迁移开始时，纤维状网络呈现出一种优选的中外侧方向。因此，可以得出结论，这种纤维排列影响细胞迁移方向。
