L-尿苷：作为酶底物的合成及特性
L-uridine: synthesis and behavior as enzyme substrate.
作者信息
Wu A F, Chargaff E
出版信息
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1222-6. doi: 10.1073/pnas.63.4.1222.
L-Uridine, the enantiomer of the normal RNA constituent D-uridine, was synthesized from L-ribose through coupling with bis(trimethylsilyl)-uracil. The synthetic product had the expected chemical and physical characteristics. When used as the acceptor for phosphate transfer by the nucleoside phosphotransferase of carrot, L-uridine is converted to 5'-L-uridylic acid. The Michaelis constants K(m) are 28 x 10(-3)M for L-uridine, 5 x 10(-3)M for D-uridine. The nucleoside phosphotransferase of human prostate, which phosphorylates D-uridine in the 5', 3', or 2' positions, fails to transfer phosphate to the 2' position of L-uridine, but does produce 5'-and 3'-L-uridylic acids.
L-尿苷是正常RNA成分D-尿苷的对映体，它由L-核糖与双（三甲基硅基）尿嘧啶偶联合成。合成产物具有预期的化学和物理特性。当用作胡萝卜核苷磷酸转移酶进行磷酸转移的受体时，L-尿苷会转化为5'-L-尿苷酸。米氏常数K(m)对于L-尿苷为28×10⁻³M，对于D-尿苷为5×10⁻³M。人前列腺的核苷磷酸转移酶可将D-尿苷在5'、3'或2'位磷酸化，但无法将磷酸转移至L-尿苷的2'位，不过确实能产生5'-和3'-L-尿苷酸。
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