纤维蛋白中受体交联位点的分子内定位
Intramolecular localization of the acceptor cross-linking sites in fibrin.
作者信息
Lorand L, Chenoweth D
出版信息
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1247-52. doi: 10.1073/pnas.63.4.1247.
Abstract
Using 1-(14)C-glycine ethyl ester to titrate and label the acceptor cross-linking sites in fibrin, it was possible to localize and characterize the reactivity of these sites. In terms of sulfitolyzed chain fragments, both alpha and gamma chains were shown to act as amine-acceptors, the site in gamma being more reactive. Identification and isolation of the acceptor loci were also achieved after cyanogen bromide fragmentation. It is interesting that the "N-terminal disulfide knot" portion of fibrin does not seem to contain acceptor functions.
摘要
用1-(14)C-甘氨酸乙酯滴定并标记纤维蛋白中的受体交联位点，可以定位并表征这些位点的反应活性。就亚硫酸化链片段而言，α链和γ链均显示为胺受体，γ链中的位点反应性更强。在溴化氰裂解后也实现了受体位点的鉴定和分离。有趣的是，纤维蛋白的“N端二硫键结”部分似乎不具有受体功能。
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