Wiedermann G, Müller M M, Förster O, Stemberger H
Int Arch Allergy Appl Immunol. 1975;49(6):843-54. doi: 10.1159/000231468.
The hapten density on the surface of sheep red cells, coated with benzylpenicilloic acid (BPO) was determined. This was achieved by comparison of the inhibitory capacity of the test cells with that of a standard cell preparation of known antigen density. IgG-induced hemolysis was much more affected by the recution of haptenic sites/cell than IgM-induced hemolysis. It was concluded that more than 18,000 protein islets on the sheep red cell surface bearing at least four haptenic sites were necessary for IgG-induced lysis. Target cells with 190,000 BPO groups/cell could be protected by anti-BPO-IgG against the lytic effect of simultaneously added IgM. Using target cells with 240,000 haptenic groups/cell or more a synergistic hemolytic action of IgG-and IgM-anti-BPO antibodies could be observed.
测定了用苄基青霉素酸（BPO）包被的绵羊红细胞表面的半抗原密度。这是通过比较测试细胞与已知抗原密度的标准细胞制剂的抑制能力来实现的。与IgM诱导的溶血相比，IgG诱导的溶血受每个细胞半抗原位点减少的影响更大。得出的结论是，绵羊红细胞表面上承载至少四个半抗原位点的超过18,000个蛋白小岛对于IgG诱导的裂解是必需的。每个细胞有190,000个BPO基团的靶细胞可以被抗BPO-IgG保护，免受同时添加的IgM的裂解作用。使用每个细胞有240,000个或更多半抗原基团的靶细胞，可以观察到IgG和IgM抗BPO抗体的协同溶血作用。
