特定的核仁与核质RNA聚合酶。
Specific nucleolar and nucleoplasmic RNA polymerases.
作者信息
Roeder R G, Rutter W J
出版信息
Proc Natl Acad Sci U S A. 1970 Mar;65(3):675-82. doi: 10.1073/pnas.65.3.675.
Abstract
The DNA-dependent RNA polymerase activity present in rat liver nuclei has been solubilized and purified from whole nuclei and from subnuclear fractions. As reported earlier (Roeder, R. G., and W. J. Rutter, Nature, 224, 234 (1969)), two major chromatographically distinct enzymatic species (I and II) are present in whole nuclei. Subfractionation of whole nuclei into nucleolar and nucleoplasmic fractions had little effect on the total recovery of activity. Purified nucleoli contain predominantly polymerase I, whereas the nucleoplasmic fraction is greatly enriched for polymerase II. A third minor peak of activity has also been resolved in the nucleoplasmic preparations. We conclude that the RNA polymerases are specifically localized within the nucleus and may, therefore, play specific roles in the regulation of genetic transcription.
摘要
大鼠肝细胞核中存在的依赖DNA的RNA聚合酶活性已从完整细胞核和亚核组分中溶解并纯化出来。如先前报道（罗德，R.G.，和W.J.拉特，《自然》，224，234（1969）），完整细胞核中存在两种主要的色谱分离不同的酶种类（I和II）。将完整细胞核亚分级为核仁组分和核质组分对活性的总回收率影响很小。纯化的核仁主要含有聚合酶I，而核质组分中聚合酶II大量富集。在核质制剂中还分辨出了第三个较小的活性峰。我们得出结论，RNA聚合酶在细胞核内特异性定位，因此可能在基因转录调控中发挥特定作用。
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