Wilson L, Bryan J, Ruby A, Mazia D
Proc Natl Acad Sci U S A. 1970 Jul;66(3):807-14. doi: 10.1073/pnas.66.3.807.
We have found that vinblastine sulfate can precipitate a number of proteins in addition to microtubule protein. Those proteins which precipitate with vinblastine sulfate, all of which were derived from structure, also precipitate with Ca(2+) ions. Our results suggest that vinblastine sulfate, presumably acting as a cation, precipitates proteins by combining with sites which can also combine with Ca(2+) ions.
我们发现硫酸长春碱除了能使微管蛋白沉淀外,还能使许多其他蛋白质沉淀。所有这些能与硫酸长春碱沉淀的蛋白质均来源于细胞结构,它们也能与钙离子沉淀。我们的结果表明,硫酸长春碱可能作为一种阳离子,通过与那些也能与钙离子结合的位点相结合而使蛋白质沉淀。