Bär H P, Hechter O, Schwartz I L, Walter R
Proc Natl Acad Sci U S A. 1970 Sep;67(1):7-12. doi: 10.1073/pnas.67.1.7.
An adenyl cyclase preparation derived from epithelial cells of the urinary bladder of the toad, Bufo marinus, is described. This cyclase preparation is specifically stimulated by neurohypophyseal hormones and various synthetic analogs which evoke a hydroosmotic response in the intact bladder. The relative stimulatory effects of these compounds have been compared on the cyclase preparation and in the intact bladder. The peptide concentrations required for half-maximal stimulation (affinity) in the cell-free and intact systems were parallel; however the magnitude of stimulation produced by saturating concentrations of peptides did not correlate. Furthermore, it was found that peptide analogs which inhibit the hydroosmotic effect of [8-arginine]-vasopressin on the intact bladder also inhibit the stimulation of the toad bladder cyclase preparation by vasopressin. Prostaglandin E(1), mercaptans, and disulfides, which inhibit the hormone-induced hydroosmotic response of the intact bladder, did not antagonize the stimulation of the toad bladder cyclase preparation by vasopressin.
本文描述了一种源自海蟾蜍（Bufo marinus）膀胱上皮细胞的腺苷酸环化酶制剂。该环化酶制剂受到神经垂体激素和各种合成类似物的特异性刺激，这些物质在完整膀胱中会引发水渗透反应。已比较了这些化合物对环化酶制剂和完整膀胱的相对刺激作用。在无细胞和完整系统中，产生半最大刺激所需的肽浓度（亲和力）是平行的；然而，饱和浓度肽产生的刺激程度并不相关。此外，还发现抑制[8-精氨酸] - 血管加压素对完整膀胱水渗透作用的肽类似物，也会抑制血管加压素对蟾蜍膀胱环化酶制剂的刺激。抑制完整膀胱激素诱导的水渗透反应的前列腺素E(1)、硫醇和二硫化物，并未拮抗血管加压素对蟾蜍膀胱环化酶制剂的刺激。
