Bonmassar E, Bonmassar A, Vadlamudi S, Goldin A
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1089-95. doi: 10.1073/pnas.66.4.1089.
L1210 leukemia was transplanted serially in CDF(1) mice treated with 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide (DIC, NSC 45388). After four different lines (C lines) had been treated for several generations, a marked increase in survival time of untreated mice was observed. In contrast, mice treated with DIC or immunosuppressed with cyclophosphamide succumbed earlier with generalized leukemia. Furthermore, a C line showed unusually high sensitivity to chemotherapeutic treatment with 1,3 bis(2-chloroethyl)-1-nitrosourea. The data suggest that C lines acquired strong antigenicity for CDF(1) and DBA/2 hosts. DIC treatment may have selected highly antigenic variants or induced somatic mutations resulting in the appearance of strong new transplantation antigen(s).
将L1210白血病细胞连续移植到用5 -(3,3 - 二甲基 - 1 - 三氮烯基)咪唑 - 4 - 甲酰胺(DIC,NSC 45388)处理的CDF(1)小鼠体内。在对四个不同品系(C品系)进行了几代处理后,观察到未处理小鼠的存活时间显著增加。相比之下,用DIC处理或用环磷酰胺免疫抑制的小鼠因全身性白血病而更早死亡。此外,一个C品系对1,3 - 双(2 - 氯乙基)- 1 - 亚硝基脲的化疗治疗表现出异常高的敏感性。数据表明,C品系对CDF(1)和DBA/2宿主获得了强抗原性。DIC处理可能选择了高抗原性变体或诱导了体细胞突变,导致出现强的新移植抗原。