Fracek S, Margulis L
Cytobios. 1979;25(97):7-16.
Hydra viridis (= Chlorohydra viridissima) the freshwater coelenterate, is symbiotic. Each individual animal harbours Chlorella sp. in its endodermal cells. The symbiosis may be disestablished, the partners grown independently, and then re-established experimentally. The most effective method to produce alga-free hydra was developed by Pardy (1976). In this study algae from homogenized H. viridis, English (= European) strain, were either used directly or first grown in pure culture and then injected to re-establish the symbiosis. The uptake of algae grown in culture was compared with that of algae released directly from fresh hydra. Fewer cultured algae were taken up. Four strains were tested: English, Carolina, Frome, and Jubilee. Each takes up a characteristic number of Chlorella when injected in excess. Colchicine, nocodazole and trifluralin, were tested for their effects on either uptake or migration. Colchicine had no effect. Nocodazole and trifluralin reduced both the number of algae taken up and the rate at which they were transported to the distal region of the hydra digestive cell. The effects of these drugs tended to be proportional to the concentration between 10(-8) and 10(-5) M.
绿水螅（= 绿藻绿水螅），一种淡水腔肠动物，是共生的。每一个体动物的内胚层细胞中都寄生有小球藻属。这种共生关系可以被打破，共生伙伴独立培养，然后通过实验重新建立。帕尔迪（1976年）开发了生产无藻水螅的最有效方法。在这项研究中，来自均质化绿水螅英国（= 欧洲）菌株的藻类要么直接使用，要么先在纯培养物中培养，然后注射以重新建立共生关系。将培养的藻类的摄取情况与直接从新鲜水螅中释放的藻类的摄取情况进行了比较。摄取的培养藻类较少。测试了四个菌株：英国、卡罗来纳、弗罗姆和朱比利。当过量注射时，每个菌株摄取特定数量的小球藻。测试了秋水仙碱、诺考达唑和氟乐灵对摄取或迁移的影响。秋水仙碱没有效果。诺考达唑和氟乐灵既减少了摄取的藻类数量，也降低了它们被运输到水螅消化细胞远端区域的速度。这些药物的作用在10^(-8) 至10^(-5) M的浓度范围内往往与浓度成正比。
