• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Studies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase.多核苷酸研究，C. 由T4 - 多核苷酸连接酶催化的一种新型连接反应。
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1468-75. doi: 10.1073/pnas.67.3.1468.
2
Interaction of bacteriophage T4 RNA and DNA ligases in joining of duplex DNA at base-paired ends.噬菌体T4 RNA连接酶与DNA连接酶在碱基配对末端双链DNA连接中的相互作用。
J Biol Chem. 1977 Jun 10;252(11):3987-94.
3
T4 polynucleotide ligase catalyzed joining of short synthetic DNA duplexes at base-paired ends.T4多核苷酸连接酶催化短的合成DNA双链在碱基配对末端处的连接。
Biochemistry. 1978 Feb 21;17(4):723-9. doi: 10.1021/bi00597a026.
4
Studies on polynucleotides. CXVI. A further study of the T4 ligase-catalyzed joining of DNA at base-paired ends.多核苷酸研究。CXVI. T4连接酶催化DNA在碱基配对末端连接的进一步研究。
J Mol Biol. 1972 Dec 30;72(3):493-502. doi: 10.1016/0022-2836(72)90170-2.
5
On the fidelity of phage T4-induced polynucleotide ligase in the joining of chemically synthesized deoxyribooligonucleotides.噬菌体T4诱导的多核苷酸连接酶在化学合成脱氧核糖寡核苷酸连接中的保真度
Biochem Biophys Res Commun. 1970 May 22;39(4):631-6. doi: 10.1016/0006-291x(70)90251-2.
6
Enzymatic oligomerization of bacteriophage P22 DNA and of linear Simian virus 40 DNA.噬菌体P22 DNA和线性猿猴病毒40 DNA的酶促寡聚化
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3389-93. doi: 10.1073/pnas.69.11.3389.
7
Polynucleotide ligase-catalyzed joining of deoxyribo-oligonucleotides on ribopolynucleotide templates and of ribo-oligonucleotides on deoxyribopolynucleotide templates.多核苷酸连接酶催化脱氧核糖寡核苷酸在核糖多核苷酸模板上的连接以及核糖寡核苷酸在脱氧核糖多核苷酸模板上的连接。
Proc Natl Acad Sci U S A. 1970 Sep;67(1):68-73. doi: 10.1073/pnas.67.1.68.
8
Studies on polynucleotides. LXXXVII. The joining of short deoxyribopolynucleotides by DNA-joining enzymes.多核苷酸研究。第八十七部分。利用DNA连接酶连接短脱氧核糖多核苷酸。
Proc Natl Acad Sci U S A. 1968 May;60(1):285-92. doi: 10.1073/pnas.60.1.285.
9
Enzymic joining of polynucleotides. VII. Role of the T4-induced ligase in the formation of recombinant molecules.多核苷酸的酶促连接。VII. T4诱导的连接酶在重组分子形成中的作用。
J Mol Biol. 1969 Dec 28;46(3):467-79. doi: 10.1016/0022-2836(69)90190-9.
10
T4 endonuclease involved in repair of DNA.参与DNA修复的T4核酸内切酶。
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1839-45. doi: 10.1073/pnas.67.4.1839.

引用本文的文献

1
The phage T4 DNA ligase mediates bacterial chromosome DSBs repair as single component non-homologous end joining.噬菌体T4 DNA连接酶作为单一组分的非同源末端连接介导细菌染色体双链断裂的修复。
Synth Syst Biotechnol. 2019 May 16;4(2):107-112. doi: 10.1016/j.synbio.2019.04.001. eCollection 2019 Jun.
2
From Structure-Function Analyses to Protein Engineering for Practical Applications of DNA Ligase.从结构-功能分析到DNA连接酶实际应用的蛋白质工程
Archaea. 2015 Oct 5;2015:267570. doi: 10.1155/2015/267570. eCollection 2015.
3
Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology.古菌核酸连接酶及其在生物技术中的潜力。
Archaea. 2015 Oct 1;2015:170571. doi: 10.1155/2015/170571. eCollection 2015.
4
Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.指数级大片段引物聚合酶链式反应 (EMP) 克隆 - 无序列限制地无缝将 DNA 插入任何目标质粒中。
PLoS One. 2012;7(12):e53360. doi: 10.1371/journal.pone.0053360. Epub 2012 Dec 31.
5
The expression in E. coli of synthetic repeating polymeric genes coding for poly(L-aspartyl-L-phenylalanine).编码聚（L-天冬氨酰-L-苯丙氨酸）的合成重复多聚基因在大肠杆菌中的表达
Nucleic Acids Res. 1980 Oct 24;8(20):4575-92. doi: 10.1093/nar/8.20.4575.
6
In vitro transcription of normal, mutant, and truncated mouse alpha-globin genes.正常、突变和截短的小鼠α-珠蛋白基因的体外转录
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7132-6. doi: 10.1073/pnas.77.12.7132.
7
Synthesis, complete 1H assignments and conformations of the self-complementary hexadeoxyribonucleotide [d(CpGpApTpCpG)]2 and its fragments by high field NMR.通过高场核磁共振对自互补十六脱氧核糖核苷酸[d(CpGpApTpCpG)]2及其片段进行合成、完整的1H归属和构象分析。
Nucleic Acids Res. 1984 Mar 12;12(5):2519-33. doi: 10.1093/nar/12.5.2519.
8
Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli.大分子拥挤效应可使大鼠肝脏或大肠杆菌来源的DNA连接酶实现平端连接。
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5852-6. doi: 10.1073/pnas.80.19.5852.
9
Primary structure and genetic organization of phage T4 DNA ligase.噬菌体T4 DNA连接酶的一级结构与基因组织
Nucleic Acids Res. 1983 Oct 25;11(20):7145-56. doi: 10.1093/nar/11.20.7145.
10
Physical mapping of the Escherichia coli D-serine deaminase region: contiguity of the dsd structural and regulatory genes.大肠杆菌D-丝氨酸脱氨酶区域的物理图谱：dsd结构基因与调控基因的连续性
J Bacteriol. 1980 Apr;142(1):174-84. doi: 10.1128/jb.142.1.174-184.1980.

本文引用的文献

1
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.一种制备高比活度32-P标记三磷酸腺苷的简单方法。
Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
2
Total synthesis of the gene for an alanine transfer ribonucleic acid from yeast.酵母丙氨酸转移核糖核酸基因的全合成
Nature. 1970 Jul 4;227(5253):27-34. doi: 10.1038/227027a0.
3
Studies on polynucleotides, 88. Enzymatic joining of chemically synthesized segments corresponding to the gene for alanine-tRNA.多核苷酸研究，88. 与丙氨酸 - tRNA基因对应的化学合成片段的酶促连接
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1338-44. doi: 10.1073/pnas.60.4.1338.
4
On the fidelity of phage T4-induced polynucleotide ligase in the joining of chemically synthesized deoxyribooligonucleotides.噬菌体T4诱导的多核苷酸连接酶在化学合成脱氧核糖寡核苷酸连接中的保真度
Biochem Biophys Res Commun. 1970 May 22;39(4):631-6. doi: 10.1016/0006-291x(70)90251-2.
5
Terminal cross-linking of DNA strands by an enzyme system from Escherichia coli infected with bacteriophage T4.由感染了噬菌体T4的大肠杆菌的一种酶系统介导的DNA链末端交联。
Proc Natl Acad Sci U S A. 1970 Mar;65(3):652-9. doi: 10.1073/pnas.65.3.652.
6
Enzymatic breakage and joining of deoxyribonucleic acid. VI. Further purification and properties of polynucleotide ligase from Escherichia coli infected with bacteriophage T4.脱氧核糖核酸的酶促断裂与连接。VI. 来自感染噬菌体T4的大肠杆菌的多核苷酸连接酶的进一步纯化及性质
J Biol Chem. 1968 Sep 10;243(17):4543-55.
7
Studies on polynucleotides. LXIX. Synthetic deoxyribopolynucleotides as templates for the DNA polymerase of Escherichia coli: DNA-like polymers containing repeating trinucleotide sequences.多核苷酸研究。第六十九篇。合成脱氧核糖多核苷酸作为大肠杆菌DNA聚合酶的模板：含有重复三核苷酸序列的类DNA聚合物。
J Mol Biol. 1967 Jul 28;27(2):237-63. doi: 10.1016/0022-2836(67)90018-6.

多核苷酸研究，C. 由T4 - 多核苷酸连接酶催化的一种新型连接反应。

Studies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase.

作者信息

Sgaramella V, Van de Sande J H, Khorana H G

出版信息

Proc Natl Acad Sci U S A. 1970 Nov;67(3):1468-75. doi: 10.1073/pnas.67.3.1468.

DOI:10.1073/pnas.67.3.1468
PMID:5274471
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC283376/
Abstract

The polynucleotide ligase isolated from T4-infected Escherichia coli was previously shown to bring about repair of breaks in the single strands of bihelical DNA. The present work shows that the enzyme can also catalyze the joining of DNA duplexes at their base-paired ends. This novel reaction occurs when the deoxynucleoside at a 5'-end carries a phosphate group and the complementary deoxynucleoside opposite to it carries a 3'-hydroxyl group. The consequence is the lengthening of the original duplex to form dimers or oligomers depending upon whether one or both ends are base-paired.

摘要

先前已表明，从感染T4噬菌体的大肠杆菌中分离出的多核苷酸连接酶能够修复双螺旋DNA单链中的断裂处。目前的研究表明，该酶还能催化DNA双链在其碱基配对末端的连接。当5'-末端的脱氧核苷带有磷酸基团，且与之相对的互补脱氧核苷带有3'-羟基时，就会发生这种新反应。其结果是，根据一端还是两端进行碱基配对，原始双链会延长形成二聚体或寡聚体。