用[203汞]硫柳汞对大鼠肝脏线粒体中的一种磷酸盐转运蛋白进行特异性标记。

Specific labeling of a phosphate-transporting protein from rat-liver mitochondria by [203Hg]mersalyl.

Hofmann H D, Kadenbach B

Eur J Biochem. 1979 Dec 17;102(2):605-13. doi: 10.1111/j.1432-1033.1979.tb04278.x.

Abstract

A highly specific labeling of a phosphate-transporting protein from rat-liver mitochondria was obtained with [203Hg]mersalyl under the following conditions: (a) labeling of mitoplasts, isolated by the French-Press procedure; (b) washing of the isolated inner membranes with NaCl/octylglucoside. 2. The concentration-dependent labeling of the protein with mersalyl showed a biphasic saturation which correlated well with the protection and inhibition of phosphate transport. 3. The molecular weight (Mr 31000) and the amount of labeled carrier protein (24 nmol/g mitochondrial protein) at 100% protection correlated well with published data from the literature. 4. Gel filtration of the labeled protein in the presence of non-ionic detergents showed a molecular weight of at least twice of that obtained by gel electrophoresis in the presence of sodium dodecylsulfate.

摘要

在以下条件下，用[203Hg]汞撒利获得了大鼠肝线粒体磷酸转运蛋白的高度特异性标记：(a) 用法国压榨法分离的线粒体质体的标记；(b) 用NaCl/辛基葡糖苷洗涤分离的内膜。2. 汞撒利对该蛋白的浓度依赖性标记呈现双相饱和，这与磷酸盐转运的保护和抑制密切相关。3. 在100%保护时，标记载体蛋白的分子量（Mr 31000）和量（24 nmol/g线粒体蛋白）与文献中已发表的数据高度吻合。4. 在非离子去污剂存在下对标记蛋白进行凝胶过滤，显示其分子量至少是在十二烷基硫酸钠存在下通过凝胶电泳获得的分子量的两倍。