Schiff L J, Byrne M M, Ketels K V, Brown W T, Graham J A
Differentiation. 1979;15(1):49-55. doi: 10.1111/j.1432-0436.1979.tb01033.x.
The ultrastructural surface features of tracheal epithelium at various times of development in organ culture were compared with those in the trachea of Syrian golden hamsters of similar age using scanning electron microscopy. Whole tracheal organ cultures, prepared from 3-day-old hamsters, were maintained in HEPES buffered CMRL 1066 medium with 0.2% bovine serum albumin. The ventral epithelial surface of trachea from 3-day-old animals was characterized by numerous microvillous cells, occasional well-developed cilia, and cells containing short cilia representing various stages of ciliary differentiation. After seven days in culture, an increased number of ciliated cells as well as developing cilia were seen. The epithelium after 14 days in culture appeared to have equal numbers of ciliated and microvillous cells, a pattern strikingly similar to that observed in vivo. After 21 days in culture, groups of well-developed cilia were interspersed with nonciliated cells covered with short, poorly developed surface microvilli. A similar pattern was found in the trachea of comparable age (24 days), with the exception of the microvillous cells; many being dome-shaped containing cluster of microvilli with prominent outlines. The tracheal organ culture, as developed in this investigation, appears to represent an excellent model for studying age-related effects of toxic and infectious agents on ciliated epithelial cells.
运用扫描电子显微镜，将气管上皮在器官培养不同发育阶段的超微结构表面特征，与相似年龄叙利亚金黄地鼠气管的特征进行比较。从3日龄地鼠制备的整个气管器官培养物，在含有0.2%牛血清白蛋白的HEPES缓冲CMRL 1066培养基中维持培养。3日龄动物气管的腹侧上皮表面特征为有大量微绒毛细胞、偶尔有发育良好的纤毛，以及含有代表纤毛分化不同阶段的短纤毛的细胞。培养7天后，可见纤毛细胞数量增加以及纤毛在发育。培养14天后的上皮似乎有相等数量的纤毛细胞和微绒毛细胞，这一模式与在体内观察到的惊人相似。培养21天后，成群发育良好的纤毛与覆盖着短而发育不良的表面微绒毛的无纤毛细胞相间分布。在可比年龄（24天）的地鼠气管中发现了类似模式，但微绒毛细胞除外；许多微绒毛细胞呈穹顶状，含有轮廓突出的微绒毛簇。本研究中所建立的气管器官培养，似乎是研究有毒和感染性因子对纤毛上皮细胞的年龄相关影响的极佳模型。
