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通过圆盘电泳法鉴定蠕虫种类。

Identification of helminth species by means of disc electrophoresis.

作者信息

Mikulíková L

出版信息

Folia Parasitol (Praha). 1979;26(2):111-22.

PMID:527899
Abstract

Soluble proteins of 25 helminth species of the classes Trematoda, Cestoidea and Nematoda, were separated by disc electrophoresis using polyacrylamide gel columns. Differences between the species were investigated on the basis of Rm values of the bands. Protein spectra were complemented by the detection of lipoproteins and glycoproteins and by identification of LDH, SHD, peroxidase, esterase and alkaline phosphatase. On the basis of comparison of protein spectra of parasitic worms belonging to three taxonomic classes it was found by means of numerical taxonomy that individual classes are characterized by a certain number of proteins of the same migration properties.

摘要

利用聚丙烯酰胺凝胶柱圆盘电泳法,对吸虫纲、绦虫纲和线虫纲的25种蠕虫的可溶性蛋白质进行了分离。根据条带的相对迁移率(Rm)值研究了不同物种之间的差异。通过检测脂蛋白和糖蛋白以及鉴定乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SHD)、过氧化物酶、酯酶和碱性磷酸酶,对蛋白质谱进行了补充。通过数值分类法,在比较三个分类纲的寄生虫蛋白质谱的基础上发现,各个纲都具有一定数量迁移特性相同的蛋白质。

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