Hildebrand J, Stryckmans P, Stoffyn P
J Lipid Res. 1971 May;12(3):361-6.
Neutral lipids, free and total cholesterol, glycolipids, and phospholipids were determined in 20 preparations of leukocytes distributed in four groups. Group I consisted of leukocytes from nonleukemic patients; group II, from patients with chronic myelogenous leukemia; group III, from patients with chronic lymphocytic leukemia; and group IV, from patients with acute leukemia. Two neutral glycolipids were found in nonleukemic mixed leukocyte populations. They were identified as glucosylceramide and lactosylceramide. The same glycolipids were also present in leukemic cells, but striking differences in glycolipid composition were found in various types of leukocytes. Glycolipids accounted for 8.9-12.6% of the total lipids in leukocytes from group I, 11.4-20.4% in group II, 1.2-1.6% in group III, and 0.5-4.9% in group IV. Glucosylceramide was the only glycolipid found in seven out of eight analyzed samples of lymphocytes, both normal and leukemic. Lactosylceramide was the major glycolipid in preparations consisting mainly of polymorphonuclear, myeloid, and blastic cells. Only lactosylceramide was found in platelets, where its concentration was about 100 times lower than in mixed leukocyte populations.
在分为四组的20份白细胞制剂中测定了中性脂质、游离胆固醇和总胆固醇、糖脂及磷脂。第一组由非白血病患者的白细胞组成；第二组来自慢性粒细胞白血病患者；第三组来自慢性淋巴细胞白血病患者；第四组来自急性白血病患者。在非白血病混合白细胞群体中发现了两种中性糖脂。它们被鉴定为葡糖神经酰胺和乳糖神经酰胺。白血病细胞中也存在相同的糖脂，但在不同类型的白细胞中发现糖脂组成存在显著差异。糖脂在第一组白细胞总脂质中占8.9 - 12.6%，第二组中占11.4 - 20.4%，第三组中占1.2 - 1.6%，第四组中占0.5 - 4.9%。在分析的8份正常和白血病淋巴细胞样本中，有7份仅发现了葡糖神经酰胺。乳糖神经酰胺是主要由多形核细胞、髓细胞和成髓细胞组成的制剂中的主要糖脂。在血小板中仅发现了乳糖神经酰胺，其浓度比混合白细胞群体中的浓度低约100倍。
