Edelman G M, Gall W E
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1444-9. doi: 10.1073/pnas.68.7.1444.
A general method of automated multidimensional fractionation has been developed. Its basic ingredients are: (a) cascade fractionation, i.e., the sequential fractionation of components obtained from each chromatographic dimension on the same or a different dimension, (b) on-line acquisition and processing of data at each stage in the fractionation procedure, (c) a method for determining the beginning and end of each peak during column elution, and (d) automatic linkage of the successive stages in a chemical fractionation scheme based on information obtained before or during each stage. Apparatus for automatic cascade chromatography and conditional fractionation is described. The method can be extended to provide completely automatic separation of pure components from complex mixtures.
已经开发出一种自动化多维分馏的通用方法。其基本要素包括：(a) 级联分馏，即对从每个色谱维度获得的组分在相同或不同维度上进行顺序分馏；(b) 在分馏过程的每个阶段在线采集和处理数据；(c) 一种在柱洗脱过程中确定每个峰起点和终点的方法；(d) 根据每个阶段之前或期间获得的信息，在化学分馏方案中自动连接连续阶段。描述了用于自动级联色谱和条件分馏的设备。该方法可以扩展以从复杂混合物中完全自动分离出纯组分。
