Triantaphyllopoulos E
Biochem J. 1973 Aug;133(4):843-5. doi: 10.1042/bj1330843.
A method is described where leaching of the column resin by pyridine-acetate buffers is minimal and excessive rise of the base-line of the chromatogram is avoided. The method can be used to fractionate peptide mixtures at a concentration of 2-20nmol/component.
本文描述了一种方法,该方法中吡啶 - 乙酸盐缓冲液对柱树脂的洗脱作用最小,且可避免色谱图基线过度上升。该方法可用于分离浓度为2 - 20nmol/组分的肽混合物。