响尾蛇毒素两种成分的生物学作用。
Biological roles of the two components of crotoxin.
作者信息
Hendon R A, Fraenkel-Conrat H
出版信息
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1560-3. doi: 10.1073/pnas.68.7.1560.
Abstract
Crotoxin, the Brazilian rattlesnake neurotoxin, generally behaves as a homogeneous protein; however, it is a molecular complex of an acidic and a basic protein. These can be separated after alkylation or acylation of the amino groups, or on carboxymethyl cellulose at pH 4, or on DEAE-cellulose in 6 M urea. The two proteins differ greatly in composition, but one or both may exist in the form of closely related variants. Their molecular weights appear to be about 8400 and 13,000. The acidic protein lacks the hemolytic and neurotoxic activity of crotoxin and the basic protein shows only the high, indirect hemolytic activity; a mixture of the two components shows the high neurotoxicity of crotoxin.
摘要
响尾蛇毒素，即巴西响尾蛇神经毒素，通常表现为一种均质蛋白；然而，它是一种酸性蛋白与一种碱性蛋白的分子复合物。在氨基进行烷基化或酰化后，或者在pH 4的羧甲基纤维素上，或者在6M尿素中的二乙氨基乙基纤维素上，这两种蛋白可以被分离。这两种蛋白在组成上有很大差异，但其中一种或两种可能以密切相关的变体形式存在。它们的分子量似乎分别约为8400和13000。酸性蛋白缺乏响尾蛇毒素的溶血和神经毒性活性，而碱性蛋白仅表现出高的间接溶血活性；这两种成分的混合物表现出响尾蛇毒素的高神经毒性。
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本文引用的文献
1
The molecular weight of crotoxin.响尾蛇毒素的分子量。
Biochem J. 1938 Aug;32(8):1375-7. doi: 10.1042/bj0321375.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.圆盘电泳。一、背景与理论。
Ann N Y Acad Sci. 1964 Dec 28;121:321-49. doi: 10.1111/j.1749-6632.1964.tb14207.x.
4
5
6
Fractionation and composition of crotoxin.响尾蛇毒素的分级分离与成分分析。
Arch Biochem Biophys. 1956 Jan;60(1):64-73. doi: 10.1016/0003-9861(56)90397-6.
7
8
Observations on molecular weight determinations on polyacrylamide gel.聚丙烯酰胺凝胶分子量测定的观察
J Biol Chem. 1969 Sep 25;244(18):5074-80.
9
Reversible blocking of amino groups with citraconic anhydride.用柠康酸酐对氨基进行可逆封闭。
Biochem J. 1968 Sep;109(2):312-4. doi: 10.1042/bj1090312.
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