6埃分辨率下的红色糖多孢菌溶菌酶结构。

Structure of Streptomyces erythraeus lysozyme at 6 A resolution.

作者信息

Sarma R, Harada S, Tanaka N, Kakudo M, Hara S, Ikenaka T

出版信息

J Biochem. 1979 Dec;86(6):1765-71. doi: 10.1093/oxfordjournals.jbchem.a132698.

DOI:10.1093/oxfordjournals.jbchem.a132698

Abstract

A 6 A resolution electron density map has been calculated for a bacterial lysozyme produced by Streptomyces erythraeus. This lysozyme differs from the vertebrate lysozyme in its size, amino acid composition, and specificity. The structure was determined by the method of isomorphous replacement. Three heavy atom derivatives were obtained by soaking crystals of the lysozyme in HgCl2, K2PtCl4, and UO2(NO3)26H2O. The resulting electron density map clearly shows the molecular boundary. The molecule is ellipsoidal in shape with average dimensions 50 A X 35 A X 35 A. High resolution analysis and sequence analysis of the molecule are in progress.

摘要

已计算出由红链霉菌产生的一种细菌溶菌酶的6埃分辨率电子密度图。这种溶菌酶在大小、氨基酸组成和特异性方面与脊椎动物溶菌酶不同。其结构通过同晶置换法确定。通过将溶菌酶晶体浸泡在HgCl2、K2PtCl4和UO2(NO3)2·6H2O中获得了三种重原子衍生物。所得的电子密度图清楚地显示了分子边界。该分子呈椭圆形，平均尺寸为50埃×35埃×35埃。对该分子的高分辨率分析和序列分析正在进行中。