Duncan I W, Culbreth P H, Burtis C A
J Chromatogr. 1979 Mar 1;162(3):281-92. doi: 10.1016/s0378-4347(00)81515-7.
A method is described for measuring free, total, and esterified cholesterol in blood serum in which reversed-phase liquid chromatography is used and the eluate is monitored at 200 nm. The sample for total cholesterol is prepared according to the Abell-Kendall procedure, and for free cholesterol an extract of serum--isopropanol (1:5, v/v) is used. The column is a muBondapak C18, 10 micrometers, and the mobile phase for total cholesterol is isopropanol--acetonitrile (50:50, v/v); for free cholesterol, it is isopropanol--acetonitrile--water (60:30:10). An approximation of the free cholesterol, triglycerides, and individual cholesteryl esters is obtained from single chromatograms of isopropanol extracts of serum if the first mobile phase is used. In a comparison study with the Abell-Kendall method for total cholesterol, the correlation is excellent and the precision is acceptable.
描述了一种用于测量血清中游离胆固醇、总胆固醇和酯化胆固醇的方法，该方法采用反相液相色谱法，并在200nm处监测洗脱液。总胆固醇的样品按照阿贝尔 - 肯德尔方法制备，游离胆固醇的样品则使用血清 - 异丙醇（1:5，v/v）提取物。色谱柱为10微米的μBondapak C18，总胆固醇的流动相为异丙醇 - 乙腈（50:50，v/v）；游离胆固醇的流动相为异丙醇 - 乙腈 - 水（60:30:10）。如果使用第一种流动相，从血清异丙醇提取物的单次色谱图中可获得游离胆固醇、甘油三酯和各个胆固醇酯的近似值。在与阿贝尔 - 肯德尔总胆固醇测定方法的比较研究中，相关性极佳且精密度可接受。
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