Cooper S F, Elie R, Albert J M, Gravel G B, Langlois Y
J Chromatogr. 1979 May 1;163(1):47-56. doi: 10.1016/s0378-4347(00)81167-6.
A sensitive gas-liquid chromatographic technique for the quantitative analysis of SCH-12679 (d-7,8-dimethoxy-3-methyl-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine acid maleate) and its major metabolites in plasma of aggressive mental retardates receiving therapeutic doses of the medicament has been developed. The lower limits of detection are 20 ng/ml for SCH-12679, 0.5 ng/ml for 3-desmethyl SCH-12679 and 0.4 ng/ml for 7-desmethyl plus 8-desmethyl SCH-12679. SCH-12679 is estimated with a flame ionization detector. Its metabolites are quantitated using an electron-capture detector after conversion of the compounds to their heptafluorobutyryl derivatives by reaction with the appropriate anhydride. Data on plasma levels of unchanged SCH-12679, 3-desmethyl SCH-12679 and a combination of 7-desmethyl and 8-desmethyl SCH-12679 in fifteen patients treated with the medicament are presented.
已开发出一种灵敏的气液色谱技术，用于定量分析接受治疗剂量该药物的攻击性智力迟钝患者血浆中的SCH - 12679（d - 7,8 - 二甲氧基 - 3 - 甲基 - 苯基 - 2,3,4,5 - 四氢 - 1H - 3 - 苯并氮杂卓酸马来酸盐）及其主要代谢物。SCH - 12679的检测下限为20 ng/ml，3 - 去甲基SCH - 12679为0.5 ng/ml，7 - 去甲基加8 - 去甲基SCH - 12679为0.4 ng/ml。SCH - 12679用火焰离子化检测器进行测定。其代谢物在与适当酸酐反应转化为七氟丁酰衍生物后，使用电子捕获检测器进行定量。给出了15名接受该药物治疗患者血浆中未变化的SCH - 12679、3 - 去甲基SCH - 12679以及7 - 去甲基和8 - 去甲基SCH - 12679组合的水平数据。
