7,8,12-三甲基苯并(a)蒽诱发大鼠白血病。I. 脾脏和胸腺的变化。
Leukemia evoked with 7,8,12-trimethylbenz(a)anthracene in rat. I. Changes in spleen and thymus.
作者信息
Bird C, Huggins C
出版信息
J Exp Med. 1971 Nov 1;134(5):1285-97. doi: 10.1084/jem.134.5.1285.
An early sign of erythroblastic leukemia in rat was nodule formation in the spleen. Hyperplastic foci of stem cells, indistinguishable histologically from leukemic stem cells, were found in the red pulp whereas the malpighian corpuscles were uninvolved. Anemia is a normal phenomenon in immature rats and the spleen of the prepubertal rat possesses considerable hemopoietic potential. Pulse-doses of 7, 8, 12-trimethylbenz(a)anthracene prevented the physiologic hematological development of maturing rats and was associated with subsequent development of leukemic stem cells in the red pulp of the spleen. Significant enzyme changes were observed in leukemic spleens. Compared with the spleens of normal littermates, the concentration of lactate dehydrogenase rose while that of malate dehydrogenase fell; the content of alkaline phosphatase rose whereas acid phosphatase fell. Increased alkaline phosphatase activity in leukemic spleen was attributed to nonleukemic foci of myelopoiesis.
大鼠成红细胞白血病的早期迹象是脾脏中出现结节。在红髓中发现了干细胞的增生灶，其组织学特征与白血病干细胞难以区分，而脾小结未受影响。贫血在未成熟大鼠中是一种正常现象，青春期前大鼠的脾脏具有相当大的造血潜力。7, 8, 12-三甲基苯并(a)蒽的脉冲剂量可阻止成熟大鼠的生理性血液学发育，并与随后脾脏红髓中白血病干细胞的发育有关。在白血病脾脏中观察到显著的酶变化。与正常同窝幼崽的脾脏相比，乳酸脱氢酶浓度升高而苹果酸脱氢酶浓度降低；碱性磷酸酶含量升高而酸性磷酸酶含量降低。白血病脾脏中碱性磷酸酶活性的增加归因于骨髓造血的非白血病灶。
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